PhD Position - Biology - Single cell genomics of tissue regeneration
- Centre / Institution:
- Center for Genomic Regulation
- Bioinformatics expertise:
The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG shares principles of an interdisciplinary, motivated and creative scientific team that is supported by high-end and innovative technologies and a flexible and efficient administration.
In November 2013, the Centre for Genomic Regulation (CRG) received the 'HR Excellence in Research' logo from the European Commission. This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers (Charter and Code).
The newly established Single Cell Genomics of Tissue Regeneration lab at CRG Barcelona is looking for a PhD student (biology), who will enrol in the very active CRG International PhD program, which includes science and practical courses, a wide range of complementary skills training, access to many courses, mentoring via a thesis committee, and active participation in the organization of seminars, symposia and retreats.
About the lab
Research in the lab will focus on the development of cutting-edge single-cell genomic and bioinformatic tools to study the maintenance, regulation and development of adult stem cells in health and disease, with a focus on the hematopoietic system and myeloid leukemia. In the past, we have used single-cell RNA-seq to challenge the text book model of hematopoiesis (https://www.nature.com/articles/ncb3493), and we have developed integrated spatial and single-cell genomic approaches to map the organization of the hematopoietic stem cell niche (under revision). In parallel, we have developed methods to investigate mRNA isoform heterogeneity at the single cell level (https://doi.org/10.15252/msb.20156198), to study the effects of cancer mutations on gene expression (https://www.biorxiv.org/content/10.1101/500108v1), and to make single-cell RNA-seq more scalable (https://doi.org/10.1534/g3.117.300257).
In the new lab, we will build on this work in order to
- Integrate single-cell RNA-seq with lineage tracing, using naturally occurring mutations, and use these methods to investigate the developmental origin and maintenance of lymphoid immune cells.
- Further develop tools to identify cancer stem cells in leukemia by single-cell genomics, and thereby identify drug targets specific to leukemic stem cells.
- Use single cell genomics as a tool for the engineering of synthetic gene regulatory elements with high lineage specificity.
Full publications: https://scholar.google.de/citations?hl=de&user=JFRp2zoAAAAJ
Desired skills and expertise
Whom would we like to hire?
- Initial exposure to genomics and/or immunology, e.g. through internships or M.Sc. thesis research
- Solid background (equivalent to M.Sc.) in biology, biotechnology, or biochemistry.
Education and training
- University degree (M.Sc. or equivalent) in Biology, Biotechnology or Biochemistry
- Fluency in English is a must
- General wetlab skills
- Practical experience in several of the following: Single cell genomics, next generation sequencing, robotics, mouse work, cell culture and lentiviral delivery, FACS
- Knowledge of R or python is a big plus!
- Willingness to learn new skills, possibly outside of your comfort zone. We work in a highly interdisciplinary manner and your project will almost certainly entail bioinformatic data analysis.
- Motivation to commit 4 years of time to a challenging research project.
- Entrepreneurial spirit needed to help set up a new lab.
Contract duration and other benefits
- Contract duration: 4 year PhD position
- Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales.
- Target start date: 15th January, 2020
We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities.
We offer and promote a diverse and inclusive environment and welcomes applicants regardless of age, disability, gender, nationality, race, religion or sexual orientation.
The CRG is committed to reconcile a work and family life of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.
Required information and contact
All applications must be addressed to Dr. Lars Velten and be submitted throught the following link:
Deadline: September 15th, 2019